Metastatic tumor cell attachment and invasion assay utilizing vascular endothelial cell monolayers.
نویسندگان
چکیده
منابع مشابه
Interactions of tumor cells with vascular endothelial cell monolayers: a model for metastatic invasion.
The interactions of tumorigenic and nontumorigenic human and rodent cells with vascular endothelial cells and their underlying extracellular matrix were studied in culture. The abilities of various cells to attach to endothelial monolayers and cause morphologic changes, such as rupture of endothelial-endothelial cell interactions leading to retraction of endothelial cells and exposure of extrac...
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A series of 11 cloned cell lines derived from a primary, nickel-induced rat rhabdomyosarcoma was evaluated for their metastatic capacity (number of lung colonies following i.v. injection) and attachment kinetics to confluent pig endothelial cell monolayers grown in vitro. The morphology of the adhering cells was also studied by optical and scanning electron microscopy. Cells from all lines test...
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Suspensions of multicellular mammary tumor spheroids (MTS) were allowed to interact with confluent monolayers of endothelial cells cultured on top of collagen gels. A number of early and late interactions between MTS and endothelial cell monolayers occurred. The early phase was characterized by the attachment of MTS to the culture and retraction of endothelial cells near the attached spheroid. ...
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The proliferation of vascular endothelial cells (EC) is an important event in angiogenesis. The synthesis of the EC growth factor, vascular endothelial cell growth factor (VEGF), is stimulated by a variety of activators; but the effects of important vasoactive peptides are not well understood, and there are no known natural inhibitors of VEGF production. We found that the vasoactive peptides en...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1982
ISSN: 0022-1554,1551-5044
DOI: 10.1177/30.3.7061823